Semester-3

1. Isolation of chloroplasts
2. Estimation of chlorophyll
3. Measurement of Hill’s reaction
4. Isolation of phosphate solubilizers
5. Isolation of IAA producing bacteria
6. Isolation and quantification of alkaloid
7. Isolation and quantification of flavonoid

1. Isolation of DNA and gel electrophoresis
2. Using Gel Documentation System to analyze DNA
3. Random mutagenesis and screening
4. DNA amplification by PCR and its phylogenic analysis
5. Southern Transfer
6. Cot curve
7. Plasmid Isolation
8. Transformation of pBR322 in E. Coli (DH5 alpha) and its screening
9. Transformation of pUC8 and its screening
10. Restriction digestion of Genomic DNA (Software)
11. Restriction digestion of Plasmid DNA
12. RNA Isolation
13. Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction
14. Comet assay/ DNA Ligation 15. Isolation of Lambda phage

At the end of course students will be able to:
1: To understand the process of photosynthesis and to compare with different types of carbon fixation.
2: To illustrate the importance of promoting plant diversity and green cover
3: Compare and contrast the pattern of development in plants with respect to animals
4: Assessing and analyzing the effect of environmental, chemical factors on plant growth development
5: Developing the apotheosis to understand intricate behaviour of metabolic pathways in different plant types
6: Imparting the capability to use various modus operandi to augment plant health and growth
7: Enabling the use of in-age techniques for the finest production of varied plant-derived phyto-chemicals