Semester-5

By the end of the paper, a student should be able to:
1: Understand the structures and functions of enzymes and its classification
2: Relate the parameters associated with enzyme activity
3: Understand mechanisms of catalysis and the involvement of various cofactors and coenzymes in the process;
4: Know concepts on how to purify a protein using various techniques
5: Understand how the rate of reaction of an enzyme is affected by physicaland chemical factors
6: Differentiate between regulatory enzymes and non regulatory enzymes through kinetic studies
7: Appreciate experiments carried out by scientists to enable understand the pathways and cycles of metabolism

Understand the concepts behind deviations from Mendel’s Laws
2: Understand the structure of chromosomes and related inheritance to chromosomes; 3: Understand the concept of gene linkage and significance of gene mapping
4: Appreciate how evolution and population genetics go hand in hand
5: Appreciate the concept of maternal inheritance
6: Carry out pedigree analysis to study genetics of diseases
7; Look at the correlation of gene expression and development of an organism
8: Appreciate experiments carried out by scientists to enable understand genetics

By the end of the paper, a student should be able to
1. Understand the basic mechanism of RNA interference and how RNAi has been developed to be used in therapeutics
2: Assess the use of stem cells for treatment of various disorders
3: Comprehend the ethical issues related to use of stem cells
4: Appreciate how understanding of molecular mechanisms has enabled therapeutic progress towards customized and target specific regimes
5: Perceive the challenges in RNAi technology, stem cell therapy and cancer therapy 6: Appreciate the role of mechanics and engineering in enabling diagnosis and treatment
7: Recognize how Bioinformatics as a tool is leading to drug discovery and analysis of medical conditions

Clinical experiments
1. Estimation of blood glucose by Arsenomolybdate. 2. Estimation of blood urea. 3. Estimation of serum uric acid. 4. Estimation of serum creatinine. 5. Estimation of glucosamine 6. Estimation of serum phosphorus
Enzyme practicals
7. Assay of salivary amylase. 8. Effect of pH on enzyme activity 9. Effect of enzyme concentration on enzyme activity 10. Effect of substrate concentration on enzyme activity 11. Effect of a competitive inhibitor on enzyme activity 12. Extraction and partial purification of an enzyme (amylase/peroxidase)

Liver Function Tests

13. Assay of serum transaminases – SGOT 14. Assay of Serum transaminases - SGPT. 15. Estimation of Alkaline Phosphatase

Molecular biology practicals

16. Verification of Chargaff’s rule by paper/ thin layer chromatography 17. Ultraviolet absorption spectrum of DNA and RNA 18. Determination of DNA concentration by UV absorption method 19. Determination of melting temperature and GC content

Enzymology is the study of enzymes, their kinetics, structure, and function, as well as their relation to each other.

A journey through the coordinated functioning of nucleic acids and proteins to enable cells to be their best functioning self.